October 13/Granada, Spain/Women's Health Weekly -- Scientists discuss in "Prediction of Extra Virgin Olive Oil Varieties Through Their Phenolic Profile. Potential Cytotoxic Activity Against Human Breast Cancer Cells" new findings in breast cancer. "The aim of this work was to develop a rapid resolution liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry (RRLC-ESI-TOF-MS) method followed by tetrazolium salt (MTT)-based cell viability assays for qualitative and quantitative classification of extra virgin olive oil (EVOO) varieties by phenolic and other polar compound contents as well as for rapid characterization of putative cytotoxic activities against human cancer cells. Five different Spanish EVOO varieties were analyzed, and RRLC-ESI-TOF-MS method was applied for qualitative and quantitative identification of most important phenolic compounds," researchers in Granada, Spain, report.
"We finally employed MTT-based cell viability protocol to assess the effects of crude EVOO phenolic extracts (PEs) on the metabolic status of cultured SKBR3 human breast cancer cells. MTT-based cell viability assays revealed a wide range of breast cancer cytotoxic potencies among individual crude PE obtained from EVOO monovarietals," wrote J. Lozano-Sanchez and colleagues, University of Granada, Department of Analytical Chemistry.
The researchers concluded, "Remarkably, breast cancer cell sensitivity to crude EVOO-PEs was up to 12 times higher in secoiridoids enriched-PE than in secoiridoids-low/ EVOO-PE."
Lozano-Sanchez and colleagues published their study in the Journal of Agricultural and Food Chemistry ("Prediction of Extra Virgin Olive Oil Varieties Through Their Phenolic Profile. Potential Cytotoxic Activity Against Human Breast Cancer Cells," Journal of Agricultural and Food Chemistry, 2010;58(18):9942-55).
For additional information, contact J. Lozano-Sanchez, University of Granada, Dept. of Analytical Chemistry, Faculty of Sciences, Fuentenueva s, n, E 18071 Granada, Spain.
From the November 1, 2010, Prepared Foods E-dition