L-isoleucine on Obesity

March 24/Oita, Japan/Health & Medicine Week --A recent study from Oita, Japan, "investigated the effects of the branched-chain amino acid L-isoleucine (Ile) on both obesity and glucose/fat homeostasis in mice that were fed a high-fat (45% energy) diet. The mice were divided into different treatment groups and given a high-fat diet for six weeks."

"During the last four weeks, Ile was dissolved and added to the drinking water to a final concentration of 2.5%. The control mice received vehicle alone. The mice in the Ile group had an almost 6% lower body weight gain and 49% less epididymal white adipose tissue (WAT) mass with the control group (P<0.05). The hepatic and skeletal muscle triglyceride (TG) concentrations and degree of hyperinsulinemia in the Ile group mice were also lower than the control group by 38%, 47% and 39%, respectively (P<0.05). The WAT leptin concentration was also lower, whereas that of adiponectin was higher, in the Ile group compared with the control group (P<0.05). The hepatic levels of protein CD36/fatty acid translocase, PPAR alpha, and uncoupling protein (UCP) 2 and the levels of UCP3 in skeletal muscle were all greater in the Ile group than in the control mice (P<0.05). These results demonstrate that the liver and muscle TG concentrations are both lowered by Ile treatment. In addition, the PPAR alpha and UCP expression levels in the mouse tissues were greater in the Ile group compared with the controls," wrote J. Nishimura and colleagues in the medical department at Oita University.

The researchers concluded, "Our current data thus suggest that supplementation with Ile might be useful in the treatment of metabolic syndrome. J. Nutr. 140: 496-500, 2010."

Nishimura and colleagues published their study in the Journal of Nutrition ("Isoleucine Prevents the Accumulation of Tissue Triglycerides and Upregulates the Expression of PPAR alpha and Uncoupling Protein in Diet-Induced Obese Mice." Journal of Nutrition, 2010;140(3):496-500).

For additional information, contact T. Masaki, Oita University, Fac Medical, Dept. of Internal Med 1, 1-1 Idaigaoka, Oita 8795593, Japan.

From the March 29, 2010, Prepared Foods E-dition