March 4/Los Angeles/University of Southern California -- People who eat a high-protein diet during middle age are more likely to die of cancer than those who eat less protein, a new study finds.

However, for people older than 65, a moderate protein intake may actually be beneficial, and protect against frailty, the researchers said.

The researchers looked at more than 6,000 people ages 50 and older, and followed them for 18 years. They found that people ages 50-65 who ate a diet rich in animal proteins during middle age were more than four times as likely to die of cancer during the study period than those who ate a low-protein diet, according to the study published in the journal Cell Metabolism.

"Popular diets in many cases have high proteins and low sugars. They may make you lose some weight, but that's not a good diet to increase life span," said study researcher Valter Longo, professor of biology at the University of Southern California (USC) and director of the USC Longevity Institute.

The risks of a high-protein diet are even comparable to smoking, the researchers said. People who smoke are four times more likely to die of cancer compared with nonsmokers, Longo said.

"Of course we cannot be sure of the reasons, but we have a pretty good idea, based on this study and also previous studies, as to why this is happening," Longo said.

The study also found that middle-age people who ate foods rich in animal proteins -- including meat, milk and cheese -- were 75% more likely to die of any cause than those who ate a low-protein diet within the study period. The researchers defined a "high-protein" diet as deriving at least 20% of daily calories from protein, a "moderate" protein diet as deriving 10-19% of calories from protein, and a "low-protein" diet as less than 10% of calories from protein.

However, what is bad for people at one age may be healthy at another. In the study, people older than 65 were less likely to die of cancer or other causes if they consumed more protein.

"So, not all people benefit from low protein. Older people actually seem to benefit from moderate intake of protein," Longo said. Having enough protein in the diet may be important for older people to maintain a healthy weight and protect against frailty, the researchers said.

The link between high-protein intake and risk of cancer almost vanished when the researchers considered participants whose protein mainly came from plants, such as beans.

This may be because proteins in plants have a different composition and do not stimulate growth hormones as efficiently as meat proteins, Longo said.

The study suggests that the Mediterranean diet, which is low in animal protein and high in carbohydrates, may be best for extending life span, Longo said.

The researchers said they recommend eating about 0.8g of protein per kilogram of body weight every day in middle age. For example, a 130-pound person should eat about 45-50g of protein a day, with a preference for plant proteins, the researchers said.

To investigate why there may be a link between protein consumption and cancer, the researchers looked at mice that were injected with mouse skin cancer cells. They showed that a high amount of protein in the mouse diet promoted the tumor's survival and growth by increasing the levels of a growth hormone, called IGF-1.

The researchers also measured the levels of IGF-1 for 2,000 people, randomly selected among those in their study, and found that for every 10 nanograms per milliliter increase in IGF-1, those on a high-protein diet were 9% more likely to die of cancer than those on a low-protein diet.

"When you have a lot of protein, these growth factors go up, and we've shown that they help normal cells become cancer-like cells, and then they help the cells grow," Longo said.